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B/R Lab Protocols


DNA preps

"Smash-and-grab" yeast DNA prep

"NIB-and-grab" yeast DNA prep for 2-D gels

Genomic yeast DNA for 2-D gels

Yeast DNA in agarose plugs

YACs for yeast transformation

 

2-D gels

Standard 2-D gels

Fork-direction gels

 

Replication timing

Density transfer

Comparative hybridization

Transient hemimethylation

 

Transformation

Frozen competent yeast

YACs for yeast transformation

 

Measurement of mutation rate

Fluctuation analysis